描述
| Parameter | Sephadex G-25 Fine, 100 g / 25g |
|---|---|
| BioProcess resin | Yes |
| Chemical stability | Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol |
| Exclusion limit (Mr) (globular proteins) | > 8 × 10³ |
| Fractionation range (Mr) globular proteins | 800 –5 × 10³ |
| Matrix | Cross-linked dextran |
| PSDdry (within specification) | 20 µm – 80 µm |
| PSDwet | 33 µm – 140 µm (10 µm – 180 µm) |
| Recommended maximum operational flow velocity | 360 cm/h in a BPG 100/500 column with 10 cm inner diameter and 20 cm bed height (at room temperature using water) |
| Storage | 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH |
| pH stability, CIP | 2–13 |
| pH stability, operational | 2–13 |
| Swelling of dry resin | 5 – 6 mL/g |
