描述
A few cell lines that Accumax (AM-105 or AM105) has been shown to work on without harm:
hESCs, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Accumax performs exceptionally well for:
hESC culturings, analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogenetransfection, neural crest cell migration assays, cell proliferation, apoptosis, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry. Accumax is used to digest primary tissue, create single cell suspensions for cell counts, and declump cells for magnetic or flow cytometry cell sorting and removing from artificial growing matrixes.
